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Study of two different enzyme immunoassays for the detection of Mayaro virus antibodies
Mem. Inst. Oswaldo Cruz ; 84(3): 303-7, jul.-set. 1989. tab
Article en En | LILACS | ID: lil-103676
Biblioteca responsable: BR1.1
RESUMO
This paper presents the evaluation of an enzyme immunoassay in which Mayaro virus-infected cultured cells ara used as antigen (EIA-ICC) and an IgM antibody capture ELISA (MAC-ELISA) for Mayaro serologic diagnosis using 114 human sera obtained during a Mayaro outbreak occurred in Bolivia, in 1987. Results were compared with those obtained by haemagglutination-inhibition test (HAI). MAC-ELISA was the most sensitive technique for anti-Mayaro IgM detection. MAC-ELISA was twice sensitive as IgM EIA-ICC. The data shows that MAC-ELISA is a practical and valid technique for diagnosis of recent mayaro infection. IgG-ICC showed hight sensitivity and high specificity compared to HAI. The combination of anti-Mayaro IgG and IgM EIA-ICC results presented the highest sensitivity of the study. Anti-Mayaro IgG and IgM simultaneous detection by ELISA-ICC can be used for recent infection diagnosis (in spite of a less sensitive IgM detection than by MAC-ELISA), for surveillance and sero-epidemiologic studies, and for studies of IgG and IgM responses to Mayaro infection
Asunto(s)
Texto completo: 1 Índice: LILACS Asunto principal: Infecciones por Togaviridae / Alphavirus / Anticuerpos Antivirales Tipo de estudio: Diagnostic_studies Límite: Humans Idioma: En Revista: Mem. Inst. Oswaldo Cruz Asunto de la revista: MEDICINA TROPICAL / PARASITOLOGIA Año: 1989 Tipo del documento: Article
Texto completo: 1 Índice: LILACS Asunto principal: Infecciones por Togaviridae / Alphavirus / Anticuerpos Antivirales Tipo de estudio: Diagnostic_studies Límite: Humans Idioma: En Revista: Mem. Inst. Oswaldo Cruz Asunto de la revista: MEDICINA TROPICAL / PARASITOLOGIA Año: 1989 Tipo del documento: Article