A technique for cardic denervation in Megalobulimus sanctpaului (mollusca, gastropoda, pulmonata)

ABSTRACT

Cardiac nerve of Megalobulinus sanctipauli can be accessed bu making an incision of about 1 cm with the aid of scalpel introduced into the connective tissue (whitsh car) joining a fold of the anterior lobe of the digestive gland. The existence of an externally identifiable anatomic characteristic (whitish scar) faciliates access to the nerve thus preventing large hemolymph loss. The percentage of hits of the technique was evauated in two different groups (N = 14 and 17) of animals, with positive results obtained for 86% and 88%, respectively. The survival rate determined 13 days after surgery for one experimental group (N = 14 was 71%