Fructose 2,6-bisphosphate and trehalose metabolism in Saccharomyces cerevisiae
Braz. j. med. biol. res
;
22(2): 171-7, 1989. tab
Artículo
en Inglés
| LILACS
| ID: lil-105573
RESUMO
1. A regulatory mutant of Sccharomyces (fdp) unable to activate fructose 1,6-bisphosphatase present a normal response to the glucose and fructose signals as measured by trehalase activation, indicating that the inability of the strain to grow on these sugars is caused by a defect located beyond membrane interactions. 2. In vivo experiments with a mutant strain bearing a phosphoglucoisomerase gene (pgil-delta) deletion showed that activation of trehalase and deactivation of the tehalose-6-phosphate synthase complex occurred to the same extent whether glucose or fructose was used as signal. 3. These results suggest that fructose-2,6-bisphosphate is not involved in the interconversion of forms of the enzymes of trehalose metabolism. Furthermore, when fructose-2,6-bisphosphate was assayed on trehalose synthesizing activity using cell-free extracts and partially purified preparations of the complex, no effect was observed. 4. We conclude that regulation by cAMP fulfills the requirements for control of trehalose levels in Saccharomyces
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Índice:
LILACS (Américas)
Asunto principal:
Saccharomyces cerevisiae
/
Trehalosa
/
AMP Cíclico
/
Disacáridos
/
Fructosadifosfatos
/
Hexosadifosfatos
Idioma:
Inglés
Revista:
Braz. j. med. biol. res
Asunto de la revista:
Biologia
/
Medicina
Año:
1989
Tipo del documento:
Artículo
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