Effect of an essential fatty acid deficient diet on experimental infection with Trypanosoma cruzi in germefree and conventional mice
Braz. j. med. biol. res
; 25(8): 795-803, 1992. tab, ilus
Article
en En
| LILACS
| ID: lil-113571
Biblioteca responsable:
BR26.1
ABSTRACT
1) To investigate the possible role of essential fatty acid deficiency in host cell/parasite interaction, weanling germefree (GF) and conventional (CV) CFW mice maintained on an essential fatty acid-deficient (-) or a control (+) diet for 110 days were infected with Trypanosoma cruzi. 2) Blood parasitemia indicated that the disease was milder in the animals maintained on the essential fatty acid-deficient diet than in the GF and CV controls (maximum parasitemia GF+33,300,GF-26,200,CV+17,100 and CV-6,400 trypomastigotes?ml blood). 3) Survival 30 days a after infection was 12% for GF+,28% for GF-,37% for CV+ and 65% for CV- mice. 4) Linoleic and arachidonic acid levels were significantly lower in animals kept on the essential fatty acid-deficient diet (GF-28.0 ñ 9.3, 23.4 ñ 8.6; CV-37.6 ñ 5.8, 19.9 ñ 3.6) than in controls (GF+ 164 ñ 48.8, 162.6 ñ 45.8; CV+ 147.1 ñ 26.5, 107.5 ñ 23.6) confirming the deficiency. Before the infection, succinic dehydrogenase levels were higher in liver of all CV mice *4.52 ug phosphate/mg fresh tissue) than in GF mice (0.84 ug phosphate/mg fresh tissue) whereas the opposite was true for 5'-nucleotidase levels in brain and liver, respectively (GF2.84 and 3.18 ug phosphate/mg fresh tissue CV1.25 and 1.54 ug phosphate?mg fresh tissue). The disease was milder in deficient than in control animals in both the GF and CV groups on the basis of parasitemia and survival, indicating that fatty acid-deficient mice are partially protected against Chagas'disease. The mechanism underlying this phenomen requires further investigation
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Índice:
LILACS
Asunto principal:
Trypanosoma cruzi
/
Ácidos Grasos Esenciales
/
Enfermedad de Chagas
/
Vida Libre de Gérmenes
Límite:
Animals
Idioma:
En
Revista:
Braz. j. med. biol. res
Asunto de la revista:
BIOLOGIA
/
MEDICINA
Año:
1992
Tipo del documento:
Article