Human papillomavirus detection in cervical dysplasias or neoplasias and in condylomata acuminata by in situ hybridization with biotinylated DNA probes
Rev. Inst. Med. Trop. Säo Paulo
;
34(4): 309-14, jul.-ago. 1992. ilus, tab
Artículo
en Inglés
| LILACS
| ID: lil-134522
ABSTRACT
Specimens from cervical dysplasias or carcinomas and genital condylomata acuminata were retrospectively analysed by in situ hybridization (ISH) with biotinylated DNA probes for human papillomavirus (HPV) types 6, 11, 16 and 18. In the control group no case was positive for HPV DNA. In mild/moderate dysplasias, 4 cases (14%) were positive for HPV 6 or 11 and 2 cases (7%), for HPV 16. In the severe dysplasia/in situ carcinoma group, 9 cases (31%) showed presence of DNA of HPV types 16 or 18. Six invasive carcinomas (20%) were positive for HPV type 16 or 18. Among condylomata acuminata, 22 cases (73%) were positive for HPV types 6 or 11. In all ISH-positive cases only one viral type was detected. No correlation between HPV DNA positivity and histological findings of HPV infection was observed. Although less sensitive than some other molecular biology techniques, in situ hybridization with biotinylated DNA probes proved to be simple and useful for detecting and typing HPV in samples routinely received for histopathological analysis
Texto completo:
Disponible
Índice:
LILACS (Américas)
Asunto principal:
Papillomaviridae
/
ADN Viral
/
Carcinoma de Células Escamosas
/
Sondas de ADN de HPV
/
Condiloma Acuminado
/
Enfermedades del Cuello del Útero
/
Displasia del Cuello del Útero
/
Neoplasias del Cuello Uterino
/
Hibridación in Situ
Tipo de estudio:
Estudio diagnóstico
/
Estudio observacional
/
Estudio de prevalencia
/
Factores de riesgo
Límite:
Femenino
/
Humanos
País/Región como asunto:
America del Sur
/
Brasil
Idioma:
Inglés
Revista:
Rev. Inst. Med. Trop. Säo Paulo
Asunto de la revista:
Medicina Tropical
Año:
1992
Tipo del documento:
Artículo
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