Expression of Mycobacterium leprae 18-kDa antigen in yeast in a GPI-anchored form
Braz. j. med. biol. res
;
27(3): 623-6, Mar. 1994. ilus
Artículo
en Inglés
| LILACS
| ID: lil-148934
RESUMO
The 18-kDa protein from Mycobacterium leprae is a major target for the immune response in leprosy. We have developed a system to express this antigen in yeast as a fusion protein with the C-terminal region of the yeast membrane protein GAS1, which would render the recombinant protein anchored to the plasma membrane by a glycosylphosphatidylinositol (GPI) anchor. Cells lacking the GAS1 gene and transformed with the hybrid 18-kDa-GAS1 construct express a polypeptide that reacts with an 18-kDa-specific monoclonal antibody. In addition, these cells react with an alpha-CRD antibody after GPI-PLC treatment. The non-transformed cells are negative. These data indicate that our system may be suitable for the expression of foreign proteins in yeast in a GPI-anchored form
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Índice:
LILACS (Américas)
Asunto principal:
Proteínas Bacterianas
/
Glicosilfosfatidilinositoles
/
Mycobacterium leprae
Idioma:
Inglés
Revista:
Braz. j. med. biol. res
Asunto de la revista:
Biologia
/
Medicina
Año:
1994
Tipo del documento:
Artículo
/
Congreso y conferencia
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