The reaction between ABTS radical cation and antioxidants and its use to evaluate the antioxidant status of serum samples
Braz. j. med. biol. res
;
29(2): 175-83, Feb. 1996. graf, tab
Artículo
en Inglés
| LILACS
| ID: lil-161667
RESUMO
The 2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) (ABTS) radical cation can be generated by incubation of ABTS and 2,2'-azo-bis(2-amidinopropane) at 45 degrees Celsius. The ABTS radical cation is stable for several minutes at room temperature and reacts quantitatively and instantaneously with several antioxidants, such as Trolox, ascorbic acid, uric acid, cysteine, glutathione and bilirubin. In contrast, the ABTS radical cation reacts slowly with albumin. When serum is added to a solution of the ABTS radical cation, the bleaching of the radical follows biphasic kinetics, with a fast decay followed by a slow decay that takes place within several minutes. The fast decay is primarily due to uric acid, while the slow decay is related to the protein content of the sample. We propose that this procedure can provide an independent and simultaneous evaluation of the low molecular weight and protein antioxidants present in biological samples such as serum.
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Índice:
LILACS (Américas)
Asunto principal:
Ácidos Sulfónicos
/
Indicadores y Reactivos
/
Antioxidantes
Límite:
Femenino
/
Humanos
/
Masculino
Idioma:
Inglés
Revista:
Braz. j. med. biol. res
Asunto de la revista:
Biologia
/
Medicina
Año:
1996
Tipo del documento:
Artículo
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