Use of molecular probes and PCR for detection and typing of Leishmania - a mini-review
Mem. Inst. Oswaldo Cruz
;
89(3): 463-9, Jul.-Sept. 1994. ilus
Artículo
en Inglés
| LILACS
| ID: lil-164121
ABSTRACT
The use of molecular tools to detect and type Leishmania species in humans, reservoirs or sandflies has been pursued using different approaches. The polymerase chain reaction provided sensitivity to case this task, since the use of hybridization procedures alone employing specifics probes is hampered due to the low detection limit. In this report, we describe the different molecular targets used in our laboratory, aiming at the detection and specific typing of these protozoa. Different kits based on hybridization assays and PCR amplification using kinetoplast and nuclear targets are described and the results obtained from their use are reported.
Texto completo:
Disponible
Índice:
LILACS (Américas)
Asunto principal:
Reacción en Cadena de la Polimerasa
/
Leishmania
Tipo de estudio:
Estudio diagnóstico
Límite:
Animales
Idioma:
Inglés
Revista:
Mem. Inst. Oswaldo Cruz
Asunto de la revista:
Medicina Tropical
/
Parasitología
Año:
1994
Tipo del documento:
Artículo
/
Congreso y conferencia
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