Multifunctional immunological monitoring of HIV positive patients: a novel staging system
P. R. health sci. j
;
12(4): 277-81, dic. 1993.
Artículo
en Inglés
| LILACS
| ID: lil-176749
ABSTRACT
A tri-functional in vitro evaluation has been utilized to analyze peripheral blood mononuclear cells (BMNC) from HIV-infected patients, which allows for the classification of these individuals into convenient stages, according to the number of in vitro parameters affected. The classifying functional parameters are the mitochondrial metabolic activity of freshly isolated BMNC, measured by an MTT reduction assay, the detection of apoptosis in 72 hour cultures of these cells assessed by propidium iodide staining and dual parametric flow cytometric analysis, and their proliferative response to pokeweed mitogen. Our results indicate that HIV-infected patients at different stages of their clinical disease, can present dysfunctions in one, two or three of the above-mentioned parameters. Based on these results, patients can be classified into four newly-described stages which are Stage 0, including uninfected controls and all patients with unaffected parameters, and Stages 1, 2 and 3, including patients having one, two or all three parameters affected, respectively. This type of immunological evaluation and classification of HIV-infected patients has the potential of becoming a predictive tool in the longitudinal follow-up of their HIV infection
Buscar en Google
Índice:
LILACS (Américas)
Asunto principal:
Seropositividad para VIH
Tipo de estudio:
Estudio diagnóstico
/
Estudio pronóstico
Límite:
Femenino
/
Humanos
Idioma:
Inglés
Revista:
P. R. health sci. j
Asunto de la revista:
Medicina
Año:
1993
Tipo del documento:
Artículo
Similares
MEDLINE
...
LILACS
LIS