A low stringency polymerase chain reaction approach to the identification of Biomphalaria glabrata and B. tenagophila, intermediate snail hosts of Schistosoma mansoni in Brazil
Mem. Inst. Oswaldo Cruz
;
91(6): 739-44, Nov.-Dec. 1996. ilus, tab, graf
Artículo
en Inglés
| LILACS
| ID: lil-181140
RESUMO
The low stringency polymerase chain reaction (LS-PCR) with a pair of specific primers for the amplification of the 18S rRNA gene was evaluted as a means of differentiating between the two Schistosoma mansoni intermediate host species in Brazil Biomphalaria glabrata and B. tenagophila. Individual snails obtained from different states of Brazil were used and the amplification patterns obtained showed a high degree of gentic variability in these species. Nevertheless, 4 and 3 clearly defined specific diagnostic bands was observed in individuals from B. glabrata and B. tenagophila respectively. The detection of snail specific diagnostic bands suggests the possibility of reliable species differentiation at DNA level using LS-PCR.
Texto completo:
Disponible
Índice:
LILACS (Américas)
Asunto principal:
Schistosoma mansoni
/
Biomphalaria
Tipo de estudio:
Estudio diagnóstico
Límite:
Animales
País/Región como asunto:
America del Sur
/
Brasil
Idioma:
Inglés
Revista:
Mem. Inst. Oswaldo Cruz
Asunto de la revista:
Medicina Tropical
/
Parasitología
Año:
1996
Tipo del documento:
Artículo
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