Analysis of respiratory syncytial virus in clinical samples by reverse transcriptase-polymerase chain reaction restriction mapping
Mem. Inst. Oswaldo Cruz
;
92(3): 389-93, May-Jun. 1997. ilus, tab
Artículo
en Inglés
| LILACS
| ID: lil-189312
RESUMO
The aim of this study was to develop a polymerase chain reation (PCR) for detection of respiratory syncytial virus (RSV) genomes. The primers were designed from published sequences and selected from conserved regions of the genome encoding for the N protein of subgroups A and B of RSV. PCR was applied to 20 specimens from children admitted to the respirary ward of "William Soler" Pediatric Hospital in Havana City with a clinical diagnosis of bronchiolitis. The PCR was compared with viral isolation and with an indirect immunofluorescence technique that employs monoclonal antibodies of subgroups A and B. Of 20 nasopharyngeal exudates, 10 were found positive by the three assayed methods. In only two cases, samples that yielded positive RNA-PCR were found negative by indirect immunofluorescence and cell culture. Considering viral isolation as the "gold standard" technique, RNA-PCR had 100 per cent sensitivity and 80 per cent specificity. RNA-PCR is a specific and sensitive technique for the detection of the RSV genome. Technical advantages are discussed.
Texto completo:
Disponible
Índice:
LILACS (Américas)
Asunto principal:
Virus Sincitiales Respiratorios
/
Reacción en Cadena de la Polimerasa
Tipo de estudio:
Estudio diagnóstico
Límite:
Niño
/
Humanos
País/Región como asunto:
Caribe
/
Cuba
Idioma:
Inglés
Revista:
Mem. Inst. Oswaldo Cruz
Asunto de la revista:
Medicina Tropical
/
Parasitología
Año:
1997
Tipo del documento:
Artículo
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