Synergetic effect of glucose and prolactin on GLUT2 expression in cultured neonatal rat islets
Braz. j. med. biol. res
;
30(3): 359-61, Mar. 1997. ilus
Artículo
en Inglés
| LILACS
| ID: lil-191347
ABSTRACT
We studied the synergistic effect of glucose and prolactin (PRL) on insulin secretion and GLUT2 expression in cultured neonatal rat islets. After 7 days in culture, basal insulin secretion (2.8 mM glucose) was similar in control and PRL-treated islets (1.84 ñ 0.06 per cent and 2.08 ñ 0.07 per cent of the islet insulin content, respectively). At 5.6 and 22 mM glucose, insulin secretion was significantly higher in PRL-treated than in control islets, achieving 1.38 ñ 0.15 per cent and 3.09 ñ 0.21 per cent of the islet insulin content in control and 2.43 ñ 0.16 per cent and 4.31 ñ 0.24 per cent of the islet insulin content in PRL-treated islets, respectively. The expression of the glucose transporter GLUT2 in B-cell membranes was dose-dependently increased by exposure of the islet to increasing glucose concentrations. This effect was potentiated in islets cultured for 7 days in the presence of 2 mug/ml PRL. At 5.6 and 10 mM glucose, the increase in GLUT2 expression in PRL-treated islets was 75 per cent and 150 per cent higher than that registered in the respective control. The data presented here indicate that insulin secretion, induced by different concentrations of glucose, correlates well with the expression of the B-cell-specific glucose transporter GLUT2 in pancreatic islets.
Texto completo:
Disponible
Índice:
LILACS (Américas)
Asunto principal:
Prolactina
/
Proteínas de Transporte de Monosacáridos
/
Islotes Pancreáticos
/
Glucosa
Límite:
Animales
/
Recién Nacido
Idioma:
Inglés
Revista:
Braz. j. med. biol. res
Asunto de la revista:
Biologia
/
Medicina
Año:
1997
Tipo del documento:
Artículo
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