Proteolytic and partial characterization of human sperm-surface glycopeptides
Braz. j. med. biol. res
;
30(3): 387-93, Mar. 1997. tab, graf
Artículo
en Inglés
| LILACS
| ID: lil-191350
RESUMO
Sperm-surface glycopeptides were obtained from intact sperm membranes after proteolytic release by different enzymatic treatments such as autoproteolysis, trypsin, papain and pronase. Glycopeptides were isolated, their properties and composition were examined, and their monosaccharide and amino acid constituents were characterized. The monosaccharides identified were fucose, mannose, galactose, N-acetylglucosamine, and N-acetylgalactosamine, which form part of more than one type of oligosaccharide units. Autoproteolytic treatmentmainly provided O-glycosidic type oligosaccharides, while a mixture of O- and N-glycosidic oligosaccharides was obtained in variable proportions when treated with trypsin, papain or pronase. The highest degree of peptide cleavage was obtained with pronase. Despite the higher yields reached with trypsin, these glycopeptides contain the lowest percentage of oligosaccharide chains. Proteolytic treatment provides a simple, rapid procedure for the isolation of glycopeptides from the sperm surface.
Texto completo:
Disponible
Índice:
LILACS (Américas)
Asunto principal:
Péptido Hidrolasas
/
Espermatozoides
/
Glicopéptidos
/
Papaína
/
Pronasa
/
Tripsina
Tipo de estudio:
Estudio pronóstico
Límite:
Humanos
/
Masculino
Idioma:
Inglés
Revista:
Braz. j. med. biol. res
Asunto de la revista:
Biologia
/
Medicina
Año:
1997
Tipo del documento:
Artículo
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