Regulation of gamma T-cell antigen receptor expression by intracellular calcium in acute lymphoblastic leukemia cell line DND41
Arch. med. res
;
27(3): 305-10, 1996. ilus
Artículo
en Inglés
| LILACS
| ID: lil-200327
ABSTRACT
The calcium inophore, ionomycin, promotes an increase of intracellular calcium and regulates mRNA expression of gamma/delta-TcR gene in human T lymphocytes. The mechanism of this regulation is not yet clear. Thus, the regulation by intracellular calcium requires elucidation. We studied the gamma-TcR gene expression in acute lymphoblastic leukemia cell line DND41 (CD4-CD8-) by Northern blot and flow cytometric analysis. The mRNA levels of gamma-TcR increased by ionomycin, anti-CD3, and with TPA. TPA had an antagonistic effect to both ionomycin and anti-CD3. Also, TPA inhibitis the increased intracellular calcium promoted by ionomycin but not the increase promoted by antiCD3 and ionomycin. Our results suggest that intracellular calcium induces mRNA and protein expression of gamma-TcR chain. This effect is antagonized by protein kinase C-activation. Thus, we conclude that the target cells of the differential regulation on gamma-TcR mRNA expression by intracellular calcium modulators are the CD4-CD8- cells, and this is due to cytosolic calcium mobilization
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Índice:
LILACS (Américas)
Asunto principal:
ARN
/
Linfocitos T CD4-Positivos
/
Expresión Génica
/
Ionomicina
/
Línea Celular
/
Northern Blotting
/
Calcio
/
Receptores de Antígenos de Linfocitos T gamma-delta
/
Leucemia-Linfoma Linfoblástico de Células Precursoras
/
Citometría de Flujo
Idioma:
Inglés
Revista:
Arch. med. res
Asunto de la revista:
Medicina
Año:
1996
Tipo del documento:
Artículo
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