In situ hybridization and immunoperoxidase for quick detection and identification of leishmania braziliensis in tissue
Parasitol. día
;
20(3/4): 86-90, jul.-dic. 1996. ilus, tab
Artículo
en Inglés
| LILACS
| ID: lil-202465
ABSTRACT
The combined used of in situ hybridization and immunoperoxidase techniques applied to histological sections of the biopsy allowed an increment of the efficiency in the detection of Leishmania diagnosis.The molecular basis for DNA diagnosis is to allow labelled single-stranded species or strain-specific DNA sequences, selected from well characterised reference species, to find and hybridize with homologous DNA from or in, the unknown isolates of parasites. The use of immunoperoxidase assay was useful in disclosing the antigen pathways and revealed amastigotes and antigenic material in various morphological patterns
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Índice:
LILACS (Américas)
Asunto principal:
Leishmania braziliensis
/
Técnicas para Inmunoenzimas
/
Hibridación in Situ
Tipo de estudio:
Estudio diagnóstico
Límite:
Animales
País/Región como asunto:
America del Sur
/
Brasil
Idioma:
Inglés
Revista:
Parasitol. día
Asunto de la revista:
Parasitología
Año:
1996
Tipo del documento:
Artículo
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