Study of a region on yeast chromosome XIII that complements pet G199 mutants (COX7) and carries a new non-essential gene
Braz. j. med. biol. res
;
31(3): 355-63, Mar. 1998. tab, graf
Artículo
en Inglés
| LILACS
| ID: lil-212283
RESUMO
The mutants of Saccharomyces cerevisiae assigned to complementation group G199 are deficient in mitochondrial respiration and lack a functional cytochrome oxidase complex. Recombinant plasmids capable of restoring respiration were cloned by transformation of mutants of this group with a yeast genomic library. Sequencing indicated that a 2.1-kb subclone encompasses the very end (last 11 amino acids) of the PET111 gene, the COX7 gene and a new gene (YMR255W) of unknown function that potentially codes for a polypeptide of 188 amino acids (about 21.5 kDa) without significant homology to any known protein. We have shown that the respiratory defect corresponding to group G199 is complemented by plasmids carrying only the COX7 gene. The gene YMR255W was inactivated by one-step gene replacement and the disrupted strain was viable and unaffected in its ability to grow in a variety of different test media such as minimal or complete media using eight distinct carbon sources at three pH values and temperatures. Inactivation of this gene also did not affect mating or sporulation.
Texto completo:
Disponible
Índice:
LILACS (Américas)
Asunto principal:
Saccharomyces cerevisiae
/
ADN de Hongos
/
Cromosomas Fúngicos
Idioma:
Inglés
Revista:
Braz. j. med. biol. res
Asunto de la revista:
Biologia
/
Medicina
Año:
1998
Tipo del documento:
Artículo
/
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