How should PCNA be assessed? Total of stained cells or only the most intensely stainde ones?

RESUMO

Objective: This study aimed to analyse whether a marker of proliferative activity (PCNA) could provide a prognosis of tumor evolution and to determine whether different interpretation criteria could after the results. Method: The presence of PCNA in 59 patients of state II (T(2) N(0,1) M(0) mammary carcinoma was determined. Result: Numerical proportions of total and intensely stained cells were established. These data were compared with anatomopathological parameters. A significant association between higher cyclin values and worse histological and nuclear grading was encountered, particulaly in patients with a "negative axilla"using the PCNA index. Cyclin values were not significant in relation to any parameters when indices from the intensely stained cells were considered exclusively. Conclusion: Higher nuclear (NG3) and histological (HGIII) grading, associated with a high PCNA index (>50), distinguish high-risk patients, and it is more appropriate considering all the stained cells as representative of PCNA indices, thus reflecting tumor aggressiveness.