Genetic variability and identification of the intermediate snail hosts of Schistosoma mansoni
Mem. Inst. Oswaldo Cruz
;
93(supl.1): 103-10, Oct. 1998. ilus, mapas
Artículo
en Inglés
| LILACS
| ID: lil-218649
RESUMO
Studies based on shell or reproductive organ morphology and genetic considerations suggest extensive intraspecific variation in Biomphalaria snails. The high variability at the morphological and genetic levels, as well as the small size of some specimens and similarities between species complicate the correct identification of these snails. Here we review our work using methods based on polymerase chain reaction (PCR) amplification for analysis of genetic variation and identification of Biomphalaria snails from Brazil, Argentina, Uruguay and Paraguay. Arbitrarily primed-PCR revealed that the genome of B. glabrata exhibits a remarkable degree of intraspecific polymorphism. Low stringency-PCR using primers for 18S rRNA permited the identification of B. glabrata, B. tenagophila and B. occidentalis. The study of individuals obtained from geographically distinct populations exhibits significant intraspecific DNA polymorphism, however specimens from the same species, exhibit some species specific LSPs. We also showed that PCR-restriction fragment of length polymorphism of the internal transcribed spacer region of Biomphlaria rDNA, using Ddel permits the differentiation of the three intermediate hosts of Schistosoma mansoni. The molecular biological techniques used in our studies are very useful for the generation of new knowledge concerning the systematics and population genetics of Biomphalaria snails.
Texto completo:
Disponible
Índice:
LILACS (Américas)
Asunto principal:
Caracoles
/
Variación Genética
/
Biomphalaria
Tipo de estudio:
Estudio diagnóstico
Límite:
Animales
País/Región como asunto:
America del Sur
/
Argentina
/
Brasil
/
Paraguay
/
Uruguay
Idioma:
Inglés
Revista:
Mem. Inst. Oswaldo Cruz
Asunto de la revista:
Medicina Tropical
/
Parasitología
Año:
1998
Tipo del documento:
Artículo
/
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