Thyroid hormone regulates protein expression in C6 glioma cells
Braz. j. med. biol. res
;
31(10): 1281-4, Oct. 1998. tab, ilus
Artículo
en Inglés
| LILACS
| ID: lil-223989
RESUMO
Thyroid hormone (T3) is essential to normal brain development. Previously, we have shown that T3 induces cerebellar astrocyte proliferation. This effect is accompanied by alteration in glial fibrillary acidic protein (GFAP) and fibronectin organization. In the present study, we report that the C6 glioma cell line, which expresses GFAP and is classified as an undifferentiated astrocytic cell type, is a target for T3 action. The C6 monolayers were treated with 50 nM T3 for 3 days, after which the cells were maintained for 2 days without medium changes. In C6 cells, T3 induced the expression of proteins of 107, 73 and 62 kDa. The hormone also up-regulated protein bands of 100 (+50 per cent), 37 (+50 per cent) and 25.5 kDa (+50 per cent) and down-regulated proteins of 94 (-100 per cent), 86.5 (-100 per cent), 68 (-100 per cent), 60 (-100 per cent), 54 (-33 per cent), 51 (-33 per cent) and 43.5 kDa (-33 per cent). We suggest, on the basis of molecular mass, that the 54-, 51- and 43.5-kDa proteins could be the cytoskeletal proteins vimentin, GFAP and actin, respectively. The down-regulation of these proteins may be involved in the effects of thyroid hormone on C6 differentiation.
Texto completo:
Disponible
Índice:
LILACS (Américas)
Asunto principal:
Triyodotironina
/
Astrocitos
/
Proteína Ácida Fibrilar de la Glía
/
Glioma
Límite:
Animales
/
Humanos
Idioma:
Inglés
Revista:
Braz. j. med. biol. res
Asunto de la revista:
Biologia
/
Medicina
Año:
1998
Tipo del documento:
Artículo
/
Congreso y conferencia
Similares
MEDLINE
...
LILACS
LIS