Use of fluorescent probes to follow membrane traffic in nerve terminals
Braz. j. med. biol. res
; 31(11): 1491-500, Nov. 1998. graf, ilus
Article
en En
| LILACS
| ID: lil-224483
Biblioteca responsable:
BR1.1
ABSTRACT
Optical tracers in conjunction with fluorescence microscopy have become widely used to follow the movement of synaptic vesicles in nerve terminals. The present review discusses the use of these optical methods to understand the regulation of exocytosis and endocytosis of synaptic vesicles. The maintenance of neurotransmission depends on the constant recycling of synaptic vesicles and important insights have been gained by visualization of vesicles with the vital dye FM1-43. A number of questions related to the control of recycling of synaptic vesicles by prolonged stimulation and the role of calcium to control membrane internalization are now being addressed. It is expected that optical monitoring of presynaptic activity coupled to appropriate genetic models will contribute to the understanding of membrane traffic in synaptic terminals.
Texto completo:
1
Índice:
LILACS
Asunto principal:
Vesículas Sinápticas
/
Movimiento Celular
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Endocitosis
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Exocitosis
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Colorantes Fluorescentes
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Terminaciones Nerviosas
Idioma:
En
Revista:
Braz. j. med. biol. res
Asunto de la revista:
BIOLOGIA
/
MEDICINA
Año:
1998
Tipo del documento:
Article
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Congress and conference