A polymerase chain reaction-based assay to identify genotype F of hepatitis B virus
Braz. j. med. biol. res
;
32(1): 45-9, Jan. 1999. ilus
Artículo
en Inglés
| LILACS
| ID: lil-226211
RESUMO
We have developed a polymerase chain reaction (PCR) assay which distinguishes genotype F from the other genotypes of hepatitis B virus (HBV). The method was used to characterize HBV strains isolated in urban areas of the Brazilian Amazon. DNA was amplified in 54 of a total of 78 HBsAg-positive serum samples, using universal, non-genotype-specific primers. Only 4 (7.4 percent) were identified as genotype F by our genotype-specific PCR assay. This proportion is notably lower than that previously reported in Argentina, Venezuela, Peru, and Central America
Texto completo:
Disponible
Índice:
LILACS (Américas)
Asunto principal:
Amplificación de Genes
/
Virus de la Hepatitis B
/
Reacción en Cadena de la Polimerasa
/
Genotipo
Límite:
Humanos
País/Región como asunto:
America del Sur
/
Brasil
Idioma:
Inglés
Revista:
Braz. j. med. biol. res
Asunto de la revista:
Biologia
/
Medicina
Año:
1999
Tipo del documento:
Artículo
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