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A liver metalloendopeptidase which degrades the circulating hypotensive peptide hormones bradykinin and atrial natriuretic peptide
Carvalho, K. M; Nava, R. A; França, M. S. F; Medeiros, M. A. S; Camaräo, G. C; Juliano, L.
  • Carvalho, K. M; Universidade Federal do Ceará. Laboratório de Neurobiologia Molecular Humana.
  • Nava, R. A; Universidade Federal do Ceará. Laboratório de Neurobiologia Molecular Humana.
  • França, M. S. F; Universidade Federal do Ceará. Laboratório de Neurobiologia Molecular Humana.
  • Medeiros, M. A. S; Universidade Federal do Ceará. Laboratório de Neurobiologia Molecular Humana.
  • Camaräo, G. C; Universidade Federal do Ceará. Laboratório de Neurobiologia Molecular Humana.
  • Juliano, L; Universidade Federal de Säo Paulo. Departamento de Biofísica.
Braz. j. med. biol. res ; 32(1): 51-4, Jan. 1999. ilus, tab
Artículo en Inglés | LILACS | ID: lil-226212
RESUMO
A new metalloendopeptidase was purified to apparent homogeneity from a homogenate of normal human liver using successive steps of chromatography on DEAE-cellulose, hydroxyapatite and Sephacryl S-200. The purified enzyme hydrolyzed the Pro7-Phe8 bond of bradykinin and the Ser25-Tyr26 bond of atrial natriuretic peptide. No cleavage was produced in other peptide hormones such as vasopressin, oxytocin or Met- and Leu-enkephalin. This enzyme activity was inhibited by 1 mM divalent cation chelators such as EDTA, EGTA and o-phenanthroline and was insensitive to 1 µM phosphoramidon and captopril, specific inhibitors of neutral endopeptidase (EC 3.4.24.11) and angiotensin-converting enzyme (EC 3.4.15.1), respectively. With Mr 85 kDa, the enzyme exhibits optimal activity at pH 7.5. The high affinity of this endopeptidase for bradykinin (Km = 10 µM) and for atrial natriuretic peptide (Km = 5 µM) suggests that it may play a physiological role in the inactivation of these circulating hypotensive peptide hormones
Asunto(s)
Texto completo: Disponible Índice: LILACS (Américas) Asunto principal: Bradiquinina / Factor Natriurético Atrial / Metaloproteasas / Hígado Límite: Adulto / Humanos Idioma: Inglés Revista: Braz. j. med. biol. res Asunto de la revista: Biologia / Medicina Año: 1999 Tipo del documento: Artículo / Congreso y conferencia

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Texto completo: Disponible Índice: LILACS (Américas) Asunto principal: Bradiquinina / Factor Natriurético Atrial / Metaloproteasas / Hígado Límite: Adulto / Humanos Idioma: Inglés Revista: Braz. j. med. biol. res Asunto de la revista: Biologia / Medicina Año: 1999 Tipo del documento: Artículo / Congreso y conferencia