Detection of point mutations by non-isotopic single strand conformation polymorphism
Braz. j. med. biol. res
;
32(1): 55-8, Jan. 1999. graf, tab
Artículo
en Inglés
| LILACS
| ID: lil-226213
RESUMO
We have developed a procedure for nonradioactive single strand conformation polymorphism analysis and applied it to the detection of point mutations in the human tumor suppressor gene p53. The protocol does not require any particular facilities or equipment, such as radioactive handling, large gel units for sequencing, or a semiautomated electrophoresis system. This technique consists of amplification of DNA fragments by PCR with specific oligonucleotide primers, denaturation, and electrophoresis on small neutral polyacrylamide gels, followed by silver staining. The sensitivity of this procedure is comparable to other described techniques and the method is easy to perform and applicable to a variety of tissue specimens
Texto completo:
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Índice:
LILACS (Américas)
Asunto principal:
Neoplasias Gástricas
/
Neoplasias del Cuello Uterino
/
Genes p53
/
Mutación Puntual
/
Polimorfismo Conformacional Retorcido-Simple
/
Neoplasias de Cabeza y Cuello
Tipo de estudio:
Estudio diagnóstico
Límite:
Humanos
Idioma:
Inglés
Revista:
Braz. j. med. biol. res
Asunto de la revista:
Biologia
/
Medicina
Año:
1999
Tipo del documento:
Artículo
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