Expression of interferons-alpha and -beta, tumor necrosis factor-alpha and transcription factors, IRF-1 and IRF-2, in leukocytes induced during interferon production
Biol. Res
;
28(4): 283-90, 1995.
Artículo
en Inglés
| LILACS
| ID: lil-228573
ABSTRACT
Analysis of gene expression in peripheral blood lymphocytes is of special interest because it could reflect physiological conditions. We have examined the expression and compared the relative amounts of specific mRNAs for interferons (IFN-alpha and IFN-beta), tumor necrosis factor-alpha (TNF-alpha) and interferon regulatory factors (IRF-1 and IRF-2) from interferon primed and Sendai virus induced peripheral blood leukocytes. Results obtained showed that IRF-1 was highly inducible by IFN treatment, IFN-alpha, TNF-alpha and IRF-2 were weakly induced by IFN treatment, and IFN-beta was not inducible by priming the cells with recombinant human IFN-alpha 2b. The IFN-alpha, IFN-beta, IRF-2 and TNF-alpha transcripts increased upon viral infection. The IRF-1 mRNA was rapidly induced by IFN treatment and decreased after Sendai virus infection. Our results show that, in peripheral blood lymphocytes, IFN-alpha and -beta genes have a different response to IFN induction, thus suggesting different regulatory mechanisms for IFN induction of type I IFN genes in peripheral blood lymphocytes
Buscar en Google
Índice:
LILACS (Américas)
Asunto principal:
Fosfoproteínas
/
Factores de Transcripción
/
Expresión Génica
/
Factor de Necrosis Tumoral alfa
/
Interferón beta
/
Interferón-alfa
/
Proteínas de Unión al ADN
/
Leucocitos
Límite:
Humanos
Idioma:
Inglés
Revista:
Biol. Res
Asunto de la revista:
Biologia
Año:
1995
Tipo del documento:
Artículo
País de afiliación:
Brasil
Similares
MEDLINE
...
LILACS
LIS