Characterization of six rat strains (Rattus norvegicus) by mitochondrial DNA restriction fragment length polymorphism
Braz. j. med. biol. res
;
32(3): 267-73, Mar. 1999.
Artículo
en Inglés
| LILACS
| ID: lil-230451
ABSTRACT
Restriction fragment length polymorphism (RFLP) was used to examine the extent of mtDNA polymorphism among six strains of rats (Rattus norvegicus) - Wistar, Wistar Munich, Brown Norway, Wistar Kyoto, SHR and SHR-SP. A survey of 26 restriction enzymes has revealed a low level of genetic divergence among strains. The sites of cleavage by EcoRI, NcoI and XmnI were shown to be polymorphic. The use of these three enzymes allows the 6 strains to be classified into 4 haplotypes and identifies specific markers for each one. The percentage of sequence divergence among all pairs of haplotypes ranged from 0.035 to 0.33 percent, which is the result of a severe population constriction undergone by the strains. These haplotypes are easily demonstrable and therefore RFLP analysis can be employed for genetic monitoring of rats within animal facilities or among different laboratories
Texto completo:
Disponible
Índice:
LILACS (Américas)
Asunto principal:
Polimorfismo de Longitud del Fragmento de Restricción
/
ADN Mitocondrial
Límite:
Animales
Idioma:
Inglés
Revista:
Braz. j. med. biol. res
Asunto de la revista:
Biologia
/
Medicina
Año:
1999
Tipo del documento:
Artículo
País de afiliación:
Brasil
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