An improved histochemical technique for differentiating adrenaline-and noradrenaline-containing cells
Arch. med. res
;
29(1): 75-7, ene.-mar. 1998. ilus
Artículo
en Inglés
| LILACS
| ID: lil-232619
ABSTRACT
Background. Sevki's histochemical technique allows specific staining of catechomamine-containing cells, yet discrimiantion between adrenaline-(ADR-) cells and noradrenaline-(NOR-) cells is unrealiable, being based on hue differences. Methods. In this work, histochemical differentiation of ADR- and NOR-cells in rat adrenal medulla was carried out by introducing two modifications to Sevki's technique 1) employment of aged Giemsa solution, and 2) addition of an alkaline differentiating step. Results. With these changes, ADR-cell stained brown, whereas NOR-cells were deep-green, resulting in a clear-cut differentiation. Conclusions. The modified technique permits to differentiate ADR- from NOR-cells in the adrenal medulla using only a bright field microscope without any sophisticated equipment. The present procedure is inexpensive and easy to carry out
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Índice:
LILACS (Américas)
Asunto principal:
Epinefrina
/
Norepinefrina
/
Diferenciación Celular
/
Ratas Wistar
/
Médula Suprarrenal
/
Histocitoquímica
Límite:
Animales
Idioma:
Inglés
Revista:
Arch. med. res
Asunto de la revista:
Medicina
Año:
1998
Tipo del documento:
Artículo
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