Cell envelope components of Yersinia pestis grown in intraperitoneal diffusion chambers
Rev. microbiol
;
29(3): 174-8, jul.-set. 1998. ilus, graf
Artículo
en Inglés
| LILACS
| ID: lil-236203
ABSTRACT
The electrophoretic profiles binding of penicillin binding proteins (PBPs) and outer membrane proteins (OMPs) of Yersinia pestis EV 76 were determined following in vivo growth in diffusion chambers implanted in the peritoneal cavity of mice. In contrast to Y. pestis grown under in vitro conditions which activate the low calcium response (LCR) regulon there was no significant qualitative or quantitative change of the PBP profile of Y. pestis cells during growth in diffusion chambers for up to 72 h following implatation in mice. Three OMPs, with molecular weight of 100, 60 and 58 kDa, were expressed in Y. pestis cells grown for 24 h, but not at 48 h or at 72 h, in diffusion chambers. These results indicate that growth of Y. pestis in intraperitoneal diffusion chambers activates genes which might be relevant to the growth in the mammal host.
Texto completo:
Disponible
Índice:
LILACS (Américas)
Asunto principal:
Penicilinas
/
Yersinia pestis
/
Proteínas Portadoras
/
Proteínas de la Membrana
Tipo de estudio:
Investigación cualitativa
Límite:
Animales
Idioma:
Inglés
Revista:
Rev. microbiol
Asunto de la revista:
Microbiologia
Año:
1998
Tipo del documento:
Artículo
País de afiliación:
Brasil
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