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Hematoxylin: a simple, multiple-use dye for chromosome analysis
Guerra, Marcelo.
Afiliación
  • Guerra, Marcelo; Universidade Federal de Pernambuco. Centro de Ciências Biológicas. Departamento de Botânica.
Genet. mol. biol ; 22(1): 77-80, Mar. 1999. ilus
Article en En | LILACS | ID: lil-243519
Biblioteca responsable: BR1.1
RESUMO
A staining mixture of hematoxylin-iron alum combined with a strong hydrochloric hydrolysis was successfully applied for chromosome observation of several kinds of plants and some animals. Slightly different procedures were developed for different materials and objectives. For plant cells, the most important technical aspect was the use of 5 N HCl hydrolysis, which resulted in a very transparent cytoplasm, combined with an intense, specific hematoxylin stain. This technique is recommended for cytogenetical analysis in general, and it is especially indicated for practical classes, due to its simplicity and high reproducibility of results. Moreover, the deep contrast observed makes this technique very useful for sequential staining of cells previously analyzed with other stains, as well as for materials with fixation problems.
Asunto(s)
Texto completo: 1 Índice: LILACS Asunto principal: Plantas / Quirópteros / Cromosomas / Colorantes / Hematoxilina / Insectos Límite: Animals Idioma: En Revista: Genet. mol. biol Asunto de la revista: GENETICA Año: 1999 Tipo del documento: Article
Texto completo: 1 Índice: LILACS Asunto principal: Plantas / Quirópteros / Cromosomas / Colorantes / Hematoxilina / Insectos Límite: Animals Idioma: En Revista: Genet. mol. biol Asunto de la revista: GENETICA Año: 1999 Tipo del documento: Article