High quality human immunoglobulin G purified from Cohn fractions by liquid chromatography
Braz. j. med. biol. res
; 33(1): 27-30, Jan. 2000. tab
Article
en En
| LILACS
| ID: lil-252253
Biblioteca responsable:
BR1.1
ABSTRACT
In order to obtain intravenous immunoglobulin G (iv IgG) of high quality from F-I+II+III or F-II+III pastes prepared by the Cohn method, we developed a chromatography process using ion exchange gels, Q-Sepharose FF and CM-Sepharose FF, and Sephacryl S-300 gel filtration. Viral inactivation was performed by incubating the preparation with pepsin at pH 4.0 at 35oC for 18 h. The characteristics of 28 batches produced by us were yield 4.3 + or - 0.2 g/l plasma, i.e., a recovery of 39.1 + or - 1.8 percent; IgG subclasses distribution IgG1 = 58.4 percent, IgG2 = 34.8 percent, IgG3 = 4.5 percent and IgG4 = 2.3 percent; IgG size distribution was 98.4 percent monomers, 1.2 percent dimers and 0.4 percent polymers and protein aggregates; anticomplement activity was less than 0.5 CH50/mg IgG, and prekallikrein activator activity (PKA) was less than 5 IU/ml. These characteristics satisfied the requirements of the European Pharmacopoea edition, and the regulations of the Brazilian Health Ministry (M.S. Portaria No. 2, 30/10/1998)
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Índice:
LILACS
Asunto principal:
Calidad de la Atención de Salud
/
Inmunoglobulina G
/
Cromatografía Liquida
/
Inmunoglobulinas Intravenosas
Límite:
Humans
Idioma:
En
Revista:
Braz. j. med. biol. res
Asunto de la revista:
BIOLOGIA
/
MEDICINA
Año:
2000
Tipo del documento:
Article