Cloning and sequence analysis of a trypanosoma cruzi alpha-butulin cDNA
Biol. Res
;
30(4): 161-6, 1997. ilus, graf
Artículo
en Inglés
| LILACS
| ID: lil-255657
ABSTRACT
A cDNA clone derived from the Trypanosoma cruzi alpha-tubulin gene was isolated and sequenced (Tc alpha tub; L37345). Tc alpha tub revealed an 87.79 percent and an 85.36 percent identity with the DNA sequence of T. brucei and Leishmania, respectively. This clone was used to study, by Northern blots, alpha-tubulin gene expression in epimastigotes, cell-cultured derived trypomastigotes and extracellular amastigotes. alpha-tubulin MRNA levels were the same in epimastigotes and trypomastigotes, however, there was a drastic decrease in amastigotes. This clone could be useful to elucidate the regulatory mechanisms of alpha-tubulin gene expression during the differentiation of T. cruzi
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Índice:
LILACS (Américas)
Asunto principal:
Trypanosoma cruzi
/
Tubulina (Proteína)
/
ARN Mensajero
/
Homología de Secuencia de Ácido Nucleico
/
Expresión Génica
/
Clonación Molecular
/
Análisis de Secuencia de ADN
/
ADN Complementario
/
Nucleótidos
Límite:
Animales
Idioma:
Inglés
Revista:
Biol. Res
Asunto de la revista:
Biologia
Año:
1997
Tipo del documento:
Artículo
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