Low cost production and purification of polyclonal antibodies to staphylococcal enterotoxin A
Rev. microbiol
;
30(2): 120-4, abr.-jun. 1999. tab, graf
Artículo
en Portugués, Inglés
| LILACS
| ID: lil-257206
ABSTRACT
An immunization scheme for production of antiserum to staphylococcal enterotoxin A (SEA) is proposed. The reference method of Robbins and Bergdoll was modified to reduce the number of doses and the amount of toxin used per animal. The best immunization scheme used injections in days 0,8,24,59,62 and 67. The amount of toxin at each injection was 5,6,20,50,100 and 200ug, respectively. The total amount of toxin was 381ug, which corresponded to a reduction of 107ug in the amount of toxin for each animal when compared to the reference method. The average antiserum titer using the Optimum Sensitivity Plate - OSP was 160 and using ELISA the titer was 1100.000. The lack of cross-reactivity with other staphylococcal enterotoxins indicated high specificity of the antibody to SEA. The proposed immunization scheme was adequate to produce specific SEA antisera, with high titers and the aditional advantage of reducing the amount of purified SEA required for immunization.
Texto completo:
Disponible
Índice:
LILACS (Américas)
Asunto principal:
Staphylococcus aureus
/
Enterotoxinas
/
Anticuerpos Antibacterianos
Tipo de estudio:
Evaluación Económica en Salud
Límite:
Animales
Idioma:
Inglés
/
Portugués
Revista:
Rev. microbiol
Asunto de la revista:
Microbiologia
Año:
1999
Tipo del documento:
Artículo
Similares
MEDLINE
...
LILACS
LIS