Selection of Trypanosoma cruzi clonal genotypes (clonet 20 and 39) isolated from Bolivian triatomines following subculture in liquid medium
Mem. Inst. Oswaldo Cruz
;
95(5): 601-7, Sept.-Oct. 2000. tab, ilus
Artículo
en Inglés
| LILACS
| ID: lil-267884
RESUMO
Previous studies showed that two groups of Trypanosoma cruzi clonal genotypes named clonet 20 and clonet 39 were predominant in Triatoma infestans, the unique vector of Chagas disease in Bolivia. These groups of clones correspond to distinct genetic clusters. These clonets were detected in T. infestans and Rhodnius pictipes fecal samples before isolation and after culture by kDNA PCR (polymerase chain rreaction) and hybridization of the amplified products with clonet specific kDNA probes named 20 and 39 as previously reported. Forty eight T. infestans and three R. pictipes infected insects captured at random in different Bolivian departments were proceeded. As previously reported the direct identification of the two major clonets in fecal samples allowed the detection of abundant mixed infections 41 percent in the original sample, however after culture, only 6 percent of mixed infections were detected. Among the 21 parasite stocks isolated from digestive tracts where mixed infections were initially detected (clonet 20 + 39) clonet 20 alone was detected in 81 percent of them. This result clearly showed that the culture step selected clonet 20 parasites over those belonging to clonet 39. The taxonomic status of the isolated stocks was also confirmed by isoenzyme typing, and correlation was observed between clustering topology and hybridization patterns with the probes 20 and 39.
Texto completo:
Disponible
Índice:
LILACS (Américas)
Asunto principal:
Trypanosoma cruzi
/
Triatominae
/
Insectos Vectores
Límite:
Animales
País/Región como asunto:
America del Sur
/
Bolivia
Idioma:
Inglés
Revista:
Mem. Inst. Oswaldo Cruz
Asunto de la revista:
Medicina Tropical
/
Parasitología
Año:
2000
Tipo del documento:
Artículo
/
Documento de proyecto
País de afiliación:
Bolivia
/
Francia
Institución/País de afiliación:
UMR/FR
/
Universidad Mayor de San Andrés/BO
Similares
MEDLINE
...
LILACS
LIS