Arbitrarily primed PCR fingerprinting of RNA and DNA in Entamoeba histolytica
Rev. Inst. Med. Trop. Säo Paulo
;
42(5): 249-53, Sept.-Oct. 2000. ilus, tab
Artículo
en Inglés
| LILACS
| ID: lil-270225
RESUMO
Differences were detected in the gene expression of strains of E. histolytica using RNA (RAP-PCR) and DNA fingerprinting (RAPD). Analysis of the electrophoretic profiles of the gels revealed some polymorphic markers that could be used in the individual characterization of the strains. The 260 bands generated by using five different primers for RAP-PCR, as well as RAPD, were employed in the construction of dendograms. The dendogram obtained based on the RAPD products permitted the distinction of symptomatic and asymptomatic isolates, as well the correlation between the polymorphism exhibited and the virulence of the strains. The dendogram obtained for the RAP-PCR products did not show a correlation with the virulence of the strains but revealed a high degree of intraspecific transcriptional variability that could be related to other biological features, whether or not these are involved in the pathogenesis of amebiasis
Texto completo:
Disponible
Índice:
LILACS (Américas)
Asunto principal:
Reacción en Cadena de la Polimerasa
/
Dermatoglifia del ADN
/
ADN Protozoario
/
ARN Protozoario
/
Entamoeba histolytica
Límite:
Animales
Idioma:
Inglés
Revista:
Rev. Inst. Med. Trop. Säo Paulo
Asunto de la revista:
Medicina Tropical
Año:
2000
Tipo del documento:
Artículo
País de afiliación:
Brasil
Institución/País de afiliación:
Universidade Federal de Minas Gerais/BR
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