Expression of mosquito active toxin genes by a Colombian native strain of the gram-negative bacterium Asticcacaulis excentricus
Mem. Inst. Oswaldo Cruz
;
96(2): 257-63, Feb. 2001. ilus, tab
Artículo
en Inglés
| LILACS
| ID: lil-281577
RESUMO
Mosquito control with biological insecticides, such as Bacillus sp. toxins, has been used widely in many countries. However, rapid sedimentation away from the mosquito larvae feeding zone causes a low residual effect. In order to overcome this problem, it has been proposed to clone the Bacillus toxin genes in aquatic bacteria which are able to live in the upper part of the water column. Two strains of Asticcacaulis excentricus were chosen to introduce the B. sphaericus binary toxin gene and B. thuringiensis subsp. medellin cry11Bb gene cloned in suitable vectors. In feeding experiments with these aquatic bacteria, it was shown that Culex quinquefasciatus, Aedes aegypti, and Anopheles albimanus larvae were able to survive on a diet based on this wild bacterium. A. excentricus recombinant strains were able to express both genes, but the recombinant strain expressing the B. sphaericus binary toxin was toxic to mosquito larvae. Crude protease A. excentricus extracts did not degrade the Cry11Bb toxin. The flotability studies indicated that the recombinant A. excentricus strains remained in the upper part of the water column longer than the wild type Bacillus strains
Texto completo:
Disponible
Índice:
LILACS (Américas)
Asunto principal:
Toxinas Bacterianas
/
Control Biológico de Vectores
/
Bacterias Gramnegativas
/
Insectos Vectores
/
Culicidae
Límite:
Animales
País/Región como asunto:
America del Sur
/
Colombia
Idioma:
Inglés
Revista:
Mem. Inst. Oswaldo Cruz
Asunto de la revista:
Medicina Tropical
/
Parasitología
Año:
2001
Tipo del documento:
Artículo
País de afiliación:
Colombia
Institución/País de afiliación:
Corporación para Investigaciones Biológicas/CO
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