Survival of tubercle bacilli in heat-fixed and stained sputum smears
Mem. Inst. Oswaldo Cruz
;
96(2): 277-80, Feb. 2001. ilus, tab
Artículo
en Inglés
| LILACS
| ID: lil-281580
ABSTRACT
We used a slide culture technique to detect tubercle bacilli surviving in sputum smears (n=46) after conventional heat fixation and Ziehl-Neelsen staining. In all heat-fixed sputum smears, tubercle bacilli survived after time 0 (n=22), 24 h (n=7), 48 h (n=7), 72 h (n=4), and seven days (n=6). None of the stained sputum smears showed growth on slide cultures. Viable tubercle bacilli remaining in heat-fixed sputum smears for at least seven days may present an infection risk to laboratory staff. Thus, sputum smears should be stained immediately by the Ziehl-Neelsen method or stored in a safe container to avoid transmission of tuberculosis
Texto completo:
Disponible
Índice:
LILACS (Américas)
Asunto principal:
Esputo
/
Colorantes
/
Calor
/
Micobacterias no Tuberculosas
Tipo de estudio:
Estudio diagnóstico
Límite:
Humanos
Idioma:
Inglés
Revista:
Mem. Inst. Oswaldo Cruz
Asunto de la revista:
Medicina Tropical
/
Parasitología
Año:
2001
Tipo del documento:
Artículo
País de afiliación:
Brasil
Institución/País de afiliación:
UFRJ/BR
/
Universidade Estadual de Maringá/BR
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