Molecular karyotype and chromosomal localization of genes encoding ß-tubulin, cysteine proteinase, hsp 70 and actin in Trypanosoma rangeli
Mem. Inst. Oswaldo Cruz
;
96(1): 113-121, Jan. 2001. ilus, tab
Artículo
en Inglés
| LILACS
| ID: lil-281637
ABSTRACT
The molecular karyotype of nine Trypanosoma rangeli strains was analyzed by contour-clamped homogeneous electric field electrophoresis, followed by the chromosomal localization of ß-tubulin, cysteine proteinase, 70 kDa heat shock protein (hsp 70) and actin genes. The T. rangeli strains were isolated from either insects or mammals from El Salvador, Honduras, Venezuela, Colombia, Panama and southern Brazil. Also, T. cruzi CL-Brener clone was included for comparison. Despite the great similarity observed among strains from Brazil, the molecular karyotype of all T. rangeli strains analyzed revealed extensive chromosome polymorphism. In addition, it was possible to distinguish T. rangeli from T. cruzi by the chromosomal DNA electrophoresis pattern. The localization of ß-tubulin genes revealed differences among T. rangeli strains and confirmed the similarity between the isolates from Brazil. Hybridization assays using probes directed to the cysteine proteinase, hsp 70 and actin genes discriminated T. rangeli from T. cruzi, proving that these genes are useful molecular markers for the differential diagnosis between these two species. Numerical analysis based on the molecular karyotype data revealed a high degree of polymorphism among T. rangeli strains isolated from southern Brazil and strains isolated from Central and the northern South America. The T. cruzi reference strain was not clustered with any T. rangeli strain
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LILACS (Américas)
Asunto principal:
Trypanosoma
/
Cisteína Endopeptidasas
/
Proteínas Protozoarias
/
Mapeo Cromosómico
/
Actinas
/
Proteínas HSP70 de Choque Térmico
Límite:
Animales
País/Región como asunto:
America Central
/
America del Sur
/
Brasil
/
Colombia
/
El Salvador
/
Honduras
/
Panamá
/
Venezuela
Idioma:
Inglés
Revista:
Mem. Inst. Oswaldo Cruz
Asunto de la revista:
Medicina Tropical
/
Parasitología
Año:
2001
Tipo del documento:
Artículo
País de afiliación:
Brasil
Institución/País de afiliación:
Instituto Oswaldo Cruz/BR
/
Universidade Federal de Santa Catarina/BR
/
Universidade do Estado do Rio de Janeiro/BR
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