Erythrophagocytosis assay in patients with autoimmune hemolytic anemia
Medicina (B.Aires)
;
61(1): 49-52, 2001. tab, graf
Artículo
en Inglés
| LILACS
| ID: lil-286378
RESUMO
The aim of this paper is to evaluate the erythrophagocytosis assay (EA) in patients with autoimmune hemolytic anemia (AIHA). Direct antiglobulin test (DAT), indirect antiglobulin test (ITA) and EA were performed in blood samples from 46 patients with presumed AIHA. The EA was carried out incubating patients'erythocytes and peripheral blood monocyte. A total of 200 monocytes were analysed to determine the percentage of active phagocytic cells (porcentage APC). In 9 of these patients the applied treatment was evaluated by DAT,IAT and EA. In 14 transfusion requirements, the compatibility tets and EA were performed. For EA, patients'monocyte were incubated with erytrocytes from previously selected units sensitized with patients'sera. The porcentage of APC was 32.1 +/- 1.7 in 35 patients with positive DAT and 17.8 +/- 1.3 in 11 patients with negative DAT. This last value was significantly higher than that with negative controls (3.7 +/- 0.3) (p= 0.01). As regards the applied treatment, patients with a successful response (n=6) showed a significant decrease in the initial porcentage APC (31.8 +/- 1.6 to 15.3 +/- 2.4; p= 0.05) while DAT and IAT remained positive. In those patients who required blood transfusion the compatibility test were positive with all the units to be transfused, whereas the porcentage APC varied for each one. Blood units were selected according to the lower porcentage APC.
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Índice:
LILACS (Américas)
Asunto principal:
Fagocitosis
/
Eritrocitos
/
Anemia Hemolítica Autoinmune
Tipo de estudio:
Estudio diagnóstico
Límite:
Humanos
Idioma:
Inglés
Revista:
Medicina (B.Aires)
Asunto de la revista:
Medicina
Año:
2001
Tipo del documento:
Artículo
País de afiliación:
Argentina
Institución/País de afiliación:
Universidad Nacional de Rosario/AR
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