In situ hybridization of somatolactin transcripts in the pituitary glands from acclimatized carp (Cyprinus carpio)
Biol. Res
;
34(1): 7-13, 2001. ilus
Artículo
en Inglés
| LILACS
| ID: lil-288328
RESUMO
We isolated and cloned a carp somatolactin SL DNA fragment, of which 78 per cent of the nucleotides were identical to the corresponding salmon SL sequence. The results obtained upon Northern blot hybridization of carp pituitary RNA allowed the identification of two transcripts as described for other fish. When the content of SL transcripts in pituitary sections from summer- and winter-acclimatized carp was quantified by in situ hybridization assays, we found no significant differences between the two seasons. In salmonids, plasma SL reaches higher levels in summer than in winter in synchrony with the water temperature cycle; in the eurythermal carp, however, the complex adaptive responses imposed by seasonal environmental changes do not seem to include the regulation of the somatolactin detected with the probe used at the transcriptional level in pituitary glands.
Texto completo:
Disponible
Índice:
LILACS (Américas)
Asunto principal:
Hipófisis
/
Hormonas Hipofisarias
/
Transcripción Genética
/
Hibridación in Situ
/
Aclimatación
Límite:
Animales
Idioma:
Inglés
Revista:
Biol. Res
Asunto de la revista:
Biologia
Año:
2001
Tipo del documento:
Artículo
País de afiliación:
Chile
Institución/País de afiliación:
Universidad Austral de Chile/CL
/
Universidad Nacional Andrés Bello/CL
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