A alpha-glycerophosphate dehydrogenase is present in Trypanosoma cruzi glycosomes
Mem. Inst. Oswaldo Cruz
;
96(5): 697-701, July 2001. graf, tab
Artículo
en Inglés
| LILACS
| ID: lil-289360
ABSTRACT
alpha-glycerophosphate dehydrogenase (alpha-GPDH-EC.1.1.1.8) has been considered absent in Trypanosoma cruzi in contradiction with all other studied trypanosomatids. After observing that the sole malate dehydrogenase can not maintain the intraglycosomal redox balance, GPDH activity was looked for and found, although in very variable levels, in epimastigotes extracts. GPDH was shown to be exclusively located in the glycosome of T. cruzi by digitonin treatment and isopycnic centrifugation. Antibody against T. brucei GPDH showed that this enzyme seemed to be present in an essentially inactive form at the beginning of the epimastigotes growth. GPDH is apparently linked to a salicylhydroxmic-sensitive glycerophosphate reoxidizing system and plays an essential role in the glycosome redox balance
Texto completo:
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Índice:
LILACS (Américas)
Asunto principal:
Trypanosoma cruzi
/
Glicerolfosfato Deshidrogenasa
/
Microcuerpos
Límite:
Animales
Idioma:
Inglés
Revista:
Mem. Inst. Oswaldo Cruz
Asunto de la revista:
Medicina Tropical
/
Parasitología
Año:
2001
Tipo del documento:
Artículo
/
Documento de proyecto
País de afiliación:
Venezuela
Institución/País de afiliación:
Universidad de Los Andes/VE
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