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Intercellular contact-dependent survival of human A549, NCI-H596 and NCI-H520 non-small cell lung carcinoma cell lines
Castro, M. A. A; Schwartsmann, G; Moreira, J. C. F.
  • Castro, M. A. A; Universidade Luterana do Brasil. Canoas. BR
  • Schwartsmann, G; Universidade Federal do Rio Grande do Sul. Hospital de Clínicas de Porto Alegre. Porto Alegre. BR
  • Moreira, J. C. F; Universidade Federal do Rio Grande do Sul. Departamento de Bioquímica. Porto Alegre. BR
Braz. j. med. biol. res ; 34(8): 1007-1013, Aug. 2001. ilus
Artículo en Inglés | LILACS | ID: lil-290149
ABSTRACT
In the present study, we examined the relationship between cell phenotype and cell survival of three human non-small cell lung carcinoma cell lines (A549, NCI-H596 and NCI-H520). Cells in exponential growth at various densities were incubated for 24 h at 37ºC in a 5 percent CO2 humidified atmosphere and then exposed to UV radiation for 1 min (256 nm, 40 W, source-to-target distance 100 cm). After two days the surviving cells were quantified by sulforhodamine á staining and DNA fragmentation assay. The differences in UV sensitivity at 60 x 10(3) cells/cm(2) among the cell lines were not related to the proliferative state of the cells but to the extent of intercellular contact. In contrast to A549 and NCI-H596, irradiated NCI-H520 cells presented lower DNA fragmentation and an aggregated cell culture phenotype even prior to confluence, suggesting that a contact-effect mechanism provides further protection against UV radiation
Asunto(s)
Texto completo: Disponible Índice: LILACS (Américas) Asunto principal: Rayos Ultravioleta / Supervivencia Celular / Carcinoma de Pulmón de Células no Pequeñas / Neoplasias Pulmonares Límite: Humanos Idioma: Inglés Revista: Braz. j. med. biol. res Asunto de la revista: Biologia / Medicina Año: 2001 Tipo del documento: Artículo País de afiliación: Brasil Institución/País de afiliación: Universidade Federal do Rio Grande do Sul/BR / Universidade Luterana do Brasil/BR

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Texto completo: Disponible Índice: LILACS (Américas) Asunto principal: Rayos Ultravioleta / Supervivencia Celular / Carcinoma de Pulmón de Células no Pequeñas / Neoplasias Pulmonares Límite: Humanos Idioma: Inglés Revista: Braz. j. med. biol. res Asunto de la revista: Biologia / Medicina Año: 2001 Tipo del documento: Artículo País de afiliación: Brasil Institución/País de afiliación: Universidade Federal do Rio Grande do Sul/BR / Universidade Luterana do Brasil/BR