A time-dependent, two-step binding mode of the nitro dye flavianic acid to trypsin in acid media
Braz. j. med. biol. res
;
34(8): 1047-1054, Aug. 2001. ilus, tab
Artículo
en Inglés
| LILACS
| ID: lil-290155
RESUMO
Synthetic dyes bind to proteins causing selective coprecipitation of the complexes in acid aqueous solution by a process of reversible denaturation that can be used as an alternative method for protein fractionation. The events that occur before precipitation were investigated by equilibrium dialysis using bovine trypsin and flavianic acid as a model able to cause coprecipitation. A two-step mode of interaction was found to be dependent on the incubation periods allowed for binding, with pronounced binding occurring after 42 h of incubation. The first step seems to involve hydration effects and conformational changes induced by binding of the first dye molecule, following rapid denaturation due to the binding of six additional flavianate anions to the macromolecule
Texto completo:
Disponible
Índice:
LILACS (Américas)
Asunto principal:
Tripsina
/
Proteínas
/
Colorantes
Tipo de estudio:
Estudio pronóstico
Límite:
Animales
Idioma:
Inglés
Revista:
Braz. j. med. biol. res
Asunto de la revista:
Biologia
/
Medicina
Año:
2001
Tipo del documento:
Artículo
País de afiliación:
Brasil
/
Estados Unidos
Institución/País de afiliación:
Universidade Federal de Minas Gerais/BR
/
Universidade de Alfenas/BR
/
University of Minnesota/US
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