Characterization of mixed infections with different strains of bovine rotavirus in an outbreak of diarrhea in dairy herds in Goiás, Brazil
Braz. j. microbiol
;
31(2): 140-5, Apr.-Jun. 2000. ilus, tab
Artículo
en Inglés
| LILACS
| ID: lil-297653
RESUMO
Ten faecal samples of bovine rotavirus from calves less than 30 days old from an outbreak of diarrhea in Hidrolândia, Goiás, Brazil were submitted to serological and molecular characterization, using enzyme immonuassay for subgrouping and serotyping, PAGE for determination of electropherotypes and PCR for genome typing. Nine samples belonged to group A/subgroup I rotavirus one samples was group A / soubgroup non-I/non-II. Four samples were characterized as G10P[11] (B223-like), four samples showed a mixture of two rotavirus strains (G6G10 and P[5]P[11]), one sample was characterized as G6P[11] and one sample was characterized only by G serotyping/genotyping, and did not react with any primer used. Two electropherotypes were detected and both were present in the same animal. This study demonstrates that two different electropherotypes and/or serotypes of bovine rotavirus can circulate in the same outbreak.
Texto completo:
Disponible
Índice:
LILACS (Américas)
Asunto principal:
Infecciones por Rotavirus
/
Rotavirus
/
Virus de la Diarrea Viral Bovina
Límite:
Animales
País/Región como asunto:
America del Sur
/
Brasil
Idioma:
Inglés
Revista:
Braz. j. microbiol
Asunto de la revista:
Microbiologia
Año:
2000
Tipo del documento:
Artículo
País de afiliación:
Brasil
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