HHV-8 infection in patients with AIDS-related Kaposi's sarcoma in Brazil
Braz. j. med. biol. res
;
34(7): 879-886, July 2001. tab
Artículo
en Inglés
| LILACS
| ID: lil-298667
RESUMO
The aims of the present study were to determine the prevalence of human herpesvirus type 8 (HHV-8) in HIV-positive Brazilian patients with (HIV+/KS+) and without Kaposi's sarcoma (HIV+/KS-) using PCR and immunofluorescence assays, to assess its association with KS disease, to evaluate the performance of these tests in detecting HHV-8 infection, and to investigate the association between anti-HHV-8 antibody titers, CD4 counts and staging of KS disease. Blood samples from 66 patients, 39 HIV+/KS+ and 27 HIV+/KS-, were analyzed for HHV-8 viremia in peripheral blood mononuclear cells by PCR and HHV-8 antigenemia for latent and lytic infection by immunofluorescence assay. Positive samples for latent nuclear HHV-8 antigen (LNA) antibodies were titrated out from 1/100 to 1/409,600 dilution. Clinical information was collected from medical records and risk behavior was assessed through an interview. HHV-8 DNA sequences were detected by PCR in 74.3 percent of KS+ patients and in 3.7 percent of KS- patients. Serological assays were similar in detecting anti-LNA antibodies and anti-lytic antigens in sera from KS+ patients (79.5 percent) and KS- patients (18.5 percent). HHV-8 was associated with KS whatever the method used, i.e., PCR (odds ratio (OR) = 7.4, 95 percent confidence interval (CI) = 2.16-25.61) or anti-LNA and anti-lytic antibodies (OR = 17.0, 95 percentCI = 4.91-59.14). Among KS+ patients, HHV-8 titration levels correlated positively with CD4 counts (rho 0.48, P = 0.02), but not with KS staging. HHV-8 is involved in the development of KS in different geographic areas worldwide, as it is in Brazil, where HHV-8 is more frequent among HIV+ patients. KS severity was associated with immunodeficiency, but no correlation was found between HHV-8 antibody titers and KS staging
Texto completo:
Disponible
Índice:
LILACS (Américas)
Asunto principal:
Sarcoma de Kaposi
/
Infecciones Oportunistas Relacionadas con el SIDA
/
Herpesvirus Humano 8
Tipo de estudio:
Estudio de etiología
/
Estudio observacional
/
Estudio de prevalencia
/
Factores de riesgo
Límite:
Femenino
/
Humanos
/
Masculino
País/Región como asunto:
America del Sur
/
Brasil
Idioma:
Inglés
Revista:
Braz. j. med. biol. res
Asunto de la revista:
Biologia
/
Medicina
Año:
2001
Tipo del documento:
Artículo
País de afiliación:
Brasil
/
Francia
/
Reino Unido
Institución/País de afiliación:
AIDS+BR
/
Hospital Santa Rita/BR
/
Institut Pasteur/FR
/
U271 INSERM/FR
/
Universidade Federal do Espírito Santo/BR
/
University College London/GB
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