Comparison of the quantitative competitive and semiquantitative RT-PCR methods for the determination of interferon-gamma mRNA levels in AIDS-free HIV-infected individuals
Braz. j. med. biol. res
;
34(10): 1271-1275, Oct. 2001. graf
Artículo
en Inglés
| LILACS
| ID: lil-299843
RESUMO
IFN-gamma mRNA expression was evaluated in nonstimulated peripheral blood mononuclear cells (PBMC) of HIV-infected and seronegative individuals using quantitative competitive and semiquantitative RT-PCR and the sensitivity of these methods was compared. A significant correlation was found between quantitative competitive and semiquantitative RT-PCR in samples of both HIV-seronegative (P = 0.004) and HIV-infected individuals (P = 0.0004). PBMC from HIV-infected individuals presented a remarkable increase of IFN-gamma mRNA expression, as determined by both types of RT-PCR methods. Semiquantitative RT-PCR even without an internal standard is also acceptable for measuring cytokine mRNA expression, but less reliable if small amounts are quantified. Moreover, we found that increased IFN-gammamRNA expression is independent of CD4+ cell count in AIDS-free HIV-infected patients
Texto completo:
Disponible
Índice:
LILACS (Américas)
Asunto principal:
ARN Mensajero
/
Leucocitos Mononucleares
/
Infecciones por VIH
/
Interferón gamma
/
Reacción en Cadena de la Polimerasa de Transcriptasa Inversa
Tipo de estudio:
Estudio diagnóstico
/
Estudio observacional
Límite:
Humanos
Idioma:
Inglés
Revista:
Braz. j. med. biol. res
Asunto de la revista:
Biologia
/
Medicina
Año:
2001
Tipo del documento:
Artículo
País de afiliación:
Hungria
Institución/País de afiliación:
National Institute of Dermato-Venereology/HU
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