Helicobacter pylori: análisis de cagA y genotipificación de vacA en Chile: detección de una cepa s2/m1 / Helicobacter pylori: cagA status and vacA genotyping in Chile, detection of a s2/m1 strain
Rev. méd. Chile
;
129(10): 1147-1153, oct. 2001. tab, graf
Artículo
en Español
| LILACS
| ID: lil-301906
ABSTRACT
Background:
The genes cagA and vacA encode H pylori virulence factors.Aim:
To genotype these genes in H pylori strains isolated from patients with upper gastrointestinal symptoms. Material andmethods:
We studied 50 patients who underwent an upper gastrointestinal endoscopy, with positive culture for H pylori. Detection of cagA and vacA gerotyping was done using polymerase chain reactions.Results:
The gene cagA was detected in 19 samples (38 per cent). Signal sequences s1 and s2 of vacA gene were detected in 16 samples each (32 per cent). There was simultaneous amplification of s1 and s2 in 6 samples and they were not detected in 9 samples. The middle region of vacA was m1 in 9 samples, m2 in 29 samples and there was simultaneous amplification of m1 and m2 in 12 samples. In 16 samples (32 per cent), more than one type of signal sequence or medial region was detected. Of those patients in whom vacA was the only genotype detected, 15 were s2/m2, 7 were s1/m1, 4 were s1/m2 and 1 was s2/m1.Conclusions:
In these patients, the infection with cagA- H pylori strains, predominates, the prevalence of infection with s1 or s2 strains is similar and the predominant medial region is m2
Texto completo:
Disponible
Índice:
LILACS (Américas)
Asunto principal:
Helicobacter pylori
/
Genotipo
Tipo de estudio:
Estudio diagnóstico
/
Factores de riesgo
Límite:
Humanos
País/Región como asunto:
America del Sur
/
Chile
Idioma:
Español
Revista:
Rev. méd. Chile
Asunto de la revista:
Medicina
Año:
2001
Tipo del documento:
Artículo
País de afiliación:
Chile
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