An indirect immunofluorescence assay to detect antibodies against St. Louis Encephalitis virus
Rev. Inst. Med. Trop. Säo Paulo
; 43(6): 339-340, Nov.-Dec. 2001. tab
Article
en En
| LILACS
| ID: lil-303045
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BR1.1
RESUMO
An in house indirect immmunofluorescence assay ( IFA ) in relation to neutralization (NT) reference test, was assessed as a fast and cheap method to carry out serological surveys for St. Louis Encephalitis virus (SLE). Sera obtained from 213 blood donors were analyzed by both tests. The prevalence of seropositivity obtained with IFA was lower than (30.98 percent) that observed on NT (41.78 percent). The relative specificity rate of IFA was 96.77 percent whereas its relative sensitivity rate was 69.66 percent. Kappa index showed a good correlation between both tests. The results indicate that neutralization assay is still the serological test with the highest sensitivity and specificity relative rates for detecting antibodies against SLE virus. Nevertheless, the IFA could be useful as an alternative test in order to learn the circulation of the Flavivirus genus in a certain area
Texto completo:
1
Índice:
LILACS
Asunto principal:
Virus de la Encefalitis de San Luis
/
Anticuerpos Antivirales
Tipo de estudio:
Diagnostic_studies
/
Risk_factors_studies
Límite:
Humans
Idioma:
En
Revista:
Rev. Inst. Med. Trop. Säo Paulo
Asunto de la revista:
MEDICINA TROPICAL
Año:
2001
Tipo del documento:
Article