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Fingerprinting of cell lines by directed amplification of minisatellite-region DNA (DAMD)
Silva, L. M; Montes De Oca, H; Diniz, C. R; Fortes-Dias, C. L.
  • Silva, L. M; Fundaçäo Ezequiel Dias (FUNED). Centro de Pesquisa e Desenvolvimento. Belo Horizonte. BR
  • Montes De Oca, H; Fundaçäo Ezequiel Dias (FUNED). Centro de Pesquisa e Desenvolvimento. Belo Horizonte. BR
  • Diniz, C. R; Fundaçäo Ezequiel Dias (FUNED). Centro de Pesquisa e Desenvolvimento. Belo Horizonte. BR
  • Fortes-Dias, C. L; Fundaçäo Ezequiel Dias (FUNED). Centro de Pesquisa e Desenvolvimento. Belo Horizonte. BR
Braz. j. med. biol. res ; 34(11): 1405-1410, Nov. 2001. ilus, tab
Artículo en Inglés | LILACS | ID: lil-303325
RESUMO
The development of in vitro propagation of cells has been an extraordinary technical advance for several biological studies. The correct identification of the cell line used, however, is crucial, as a mistaken identity or the presence of another contaminating cell may lead to invalid and/or erroneous conclusions. We report here the application of a DNA fingerprinting procedure (directed amplification of minisatellite-region DNA), developed by Heath et al. [Nucleic Acids Research (1993) 21 5782-5785], to the characterization of cell lines. Genomic DNA of cells in culture was extracted and amplified by PCR in the presence of VNTR core sequences, and the amplicons were separated by agarose gel electrophoresis. After image capture with a digital camera, the banding profiles obtained were analyzed using a software (AnaGel) specially developed for the storage and analysis of electrophoretic fingerprints. The fingerprints are useful for construction of a data base for identification of cell lines by comparison to reference profiles as well as comparison of similar lines from different sources and periodic follow-up of cells in culture
Asunto(s)
Texto completo: Disponible Índice: LILACS (Américas) Asunto principal: Línea Celular / Dermatoglifia del ADN / Repeticiones de Minisatélite Límite: Animales / Humanos Idioma: Inglés Revista: Braz. j. med. biol. res Asunto de la revista: Biologia / Medicina Año: 2001 Tipo del documento: Artículo País de afiliación: Brasil Institución/País de afiliación: Fundaçäo Ezequiel Dias (FUNED)/BR

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Texto completo: Disponible Índice: LILACS (Américas) Asunto principal: Línea Celular / Dermatoglifia del ADN / Repeticiones de Minisatélite Límite: Animales / Humanos Idioma: Inglés Revista: Braz. j. med. biol. res Asunto de la revista: Biologia / Medicina Año: 2001 Tipo del documento: Artículo País de afiliación: Brasil Institución/País de afiliación: Fundaçäo Ezequiel Dias (FUNED)/BR