Intraspecific variation in the venom electrophoretic profile of recently captured Crotalus durissus terrificus (Laurenti, 1768) snakes
J. venom. anim. toxins
;
7(2): 276-301, 2001. ilus, tab
Artículo
en Inglés
| LILACS
| ID: lil-303718
ABSTRACT
The aim of this study was to compare individual venom samples of Crotalus durissus terrificus recently captured in the wild to evaluate possible differences in venom protein composition. Protein levels were quantified by biochemical method (Biuret) and then submitted to electrophoresis. Electrophoresis studies of native protein were performed in vertical slabs of polyacrylamide gel (PAGE), in an alkaline discontinuous buffer system, with a concentration of 10 per cent in the separation gel. SDS-PAGE was performed in PhastGel (8-25). Both gels were stained with Coomassie Blue. Gels were analyzed using the VDS-Pharmacia device. Our results indicate that all analyzed venom samples showed different protein composition, although common protein fractions were detected in some individual samples. Differences were observed between the different individual venom samples and so in the same specimen in relation to the time of collection, for both techniques used. Diet did not influence the variability of venom composition. There is a significant difference in native venom protein composition of males and females.
Texto completo:
Disponible
Índice:
LILACS (Américas)
Asunto principal:
Brasil
/
Crotalus
/
Venenos de Crotálidos
/
Electroforesis en Gel de Poliacrilamida
Límite:
Animales
País/Región como asunto:
America del Sur
/
Brasil
Idioma:
Inglés
Revista:
J. venom. anim. toxins
Asunto de la revista:
Toxicología
Año:
2001
Tipo del documento:
Artículo
País de afiliación:
Brasil
Institución/País de afiliación:
Universidade Estadual Paulista/BR
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