Intraspecific variation in neurotoxic and myotoxic activities of Bothrops neuwiedii venoms
J. venom. anim. toxins
;
8(1): 88-101, 2002. ilus, tab, graf
Artículo
en Inglés
| LILACS
| ID: lil-303724
ABSTRACT
Snake venoms frequently vary in composition. In this work, we compared the neurotoxic and myotoxic activities of 16 lots of Bothrops neuwiedii venoms from different regions of Brazil, using chick biventer cervicis preparations. The neuromuscular blockade varied from 2 per cent to 100 per cent after 120 min incubation with venoms (50µg/ml). In all cases, this blockade was irreversible and concentration-dependent; at low concentrations (10-20 µg/ml), 15 of the 16 venom lots failed to abolish responses to acetylcholine (110µM), but blocked responses to KCI (13.4mM), and induced contracture. At 5-20µg/ml, the most active venom totally blocked twitch-tension without affecting responses to acetylcholine and KCI. Polyacrylamine gel electrophoresis for basic proteins showed that the most active samples contained a band that was absent in the less active venoms. These results indicate that there may be considerable intraspecific variation in the neurotoxic activity of B. ineuwiedii venoms, whereas myotoxic activity is less variable.
Texto completo:
Disponible
Índice:
LILACS (Américas)
Asunto principal:
Brasil
/
Pollos
/
Bothrops
/
Venenos de Crotálidos
/
Miotonía
/
Sistema Nervioso
/
Neurotoxinas
Límite:
Animales
País/Región como asunto:
America del Sur
/
Brasil
Idioma:
Inglés
Revista:
J. venom. anim. toxins
Asunto de la revista:
Toxicología
Año:
2002
Tipo del documento:
Artículo
País de afiliación:
Brasil
Institución/País de afiliación:
Universidade Estadual de Campinas/BR
/
Universidade de Säo Paulo/BR
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