Additives and protein-DNA combinations modulate the humoral immune response elicited by a hepatitis C virus core-encoding plasmid in mice
Mem. Inst. Oswaldo Cruz
;
97(1): 95-99, Jan. 2002. tab, graf
Artículo
en Inglés
| LILACS
| ID: lil-306070
ABSTRACT
Humoral and cellular immune responses are currently induced against hepatitis C virus (HCV) core following vaccination with core-encoding plasmids. However, the anti-core antibody response is frequently weak or transient. In this paper, we evaluated the effect of different additives and DNA-protein combinations on the anti-core antibody response. BALB/c mice were intramuscularly injected with an expression plasmid (pIDKCo), encoding a C-terminal truncated variant of the HCV core protein, alone or combined with CaCl2, PEG 6000, Freund's adjuvant, sonicated calf thymus DNA and a recombinant core protein (Co.120). Mixture of pIDKCo with PEG 6000 and Freund's adjuvant accelerated the development of the anti-core Ab response. Combination with PEG 6000 also induced a bias to IgG2a subclass predominance among anti-core antibodies. The kinetics, IgG2a/IgG1 ratio and epitope specificity of the anti-core antibody response elicited by Co.120 alone or combined with pIDKCo was different regarding that induced by the pIDKCo alone. Our data indicate that the antibody response induced following DNA immunization can be modified by formulation strategies
Texto completo:
Disponible
Índice:
LILACS (Américas)
Asunto principal:
Plásmidos
/
Proteínas Recombinantes
/
Proteínas del Núcleo Viral
/
Hepacivirus
Límite:
Animales
Idioma:
Inglés
Revista:
Mem. Inst. Oswaldo Cruz
Asunto de la revista:
Medicina Tropical
/
Parasitología
Año:
2002
Tipo del documento:
Artículo
País de afiliación:
Cuba
Institución/País de afiliación:
Centro Nacional de Genética Médica/CU
/
Centro de Ingeniería Genética y Biotecnología/CU
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